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Bioactive Compounds

NAO
Brochure 》

Neoagarooligosaccharides (Dyne-NAO)

Introduction

Dyne Bio Inc. has developed a new bioactive compound from red algae, Gelidium elegans.


Dyne-NAO is obtained by decomposing agar which is a major component of red algae, Gelidium elegans. that is one of Korea’s main marine resources. We produce neoagarotetraose(DP4) and neoagarohexaose(DP6) through the enzymatic bio conversion process. As a new bioactive compound, we verified their effectiveness and safety. Currently we are working on developing them as food and pharmaceutical materials.

Dyne-NAO was completed safety evaluations and has significant effects on improving metabolic syndrome (obesity, diabetes and hyperlipidemia), immune modulation, moisturizing and anti-oxidant. Consequently, we are promoting business with Dyne-NAO as a high value functional biomaterial.

Structure of Dyne-NAO

Category | Compound DP4 DP6
CAS registry number 16033-31-1 25023-93-2
Molecular weight 630.547 C24H38O19
Molecular formula C24H38O19 C36H56O28

Biological efficacy

Efficacy Biological activity

Metabolic Syndrome

- Obesity
- Diabetes
- Hyperlipidemia
- Hepatic injury
• Obesity

- Reduction of the adipocytes size

- Upregulation of lipolysis related gene expression

- Downregulation of lipogenesis related gene expression

• Diabetes

- Improvement of insulin resistance

- Improvement of glycemic control

• Hyperlipidemia

- Improvement of serum lipid profiling

- Upregulation of lipid receptor related gene expression (SREBP2, PCSK9)

• Hepatic injury

- Activation of antioxidant genes via Nrf2 activation for acute liver damage

- Prevention and treatment of chronic liver injury via inhibition of
  the TFG-β/Smad pathway

Immune system
• Immune cell stimulation

- Macrophage, NK cells, T cells, etc.

• Dendritic cells maturation

- Activation of NK, Non NK immunocyte

- Identification of Toll-like Receptor (TLR)

• Inhibition of tumor growth (in vivo)
• Prevention & treatment of Microbial infection (in vivo)
• Preventing & improving sepsis (in vitro, in vivo)
• Improving & treating rheumatoid arthritis and atopic dermatitis
• Anti-viral effect against Norovirus

Patent

No Patent Registration No.
(Registration Date : DD/MM/YYYY)
1 Agar-derived neoagarooligosaccharide complex for the prevention
and treatment of obesity and/or diabetes comprising enzyme
reaction products of beta agarase DagA and agar 		Rep. of Korea 10-1632262
(15/06/2016)
Japan JP6088657
(10/02/2017)
China ZL201380059074.6
(23/06/2017)
2 Composition comprising neoagarooligosaccharide for preventing
or treating sepsis or septic shock 		Rep. of Korea 10-1675359
(07/11/2016)
U.S. 10,548,916
(04/02/2020)
China ZL 201580075360.0
(26/06/2020)
3 Composition for preventing, alleviating, or treating arthritis
or osteoporosis, containing neoagarooligosaccharide 		Rep. of Korea 10-1923062
(22/11/2018)
Japan JP6815525
(24/12/2020)
U.S. 10,933,086
(02/03/2021)
4 Mutant strain of Streptomyces coelicolor, method for producing
β-agarase using the same and method for manufacturing
neoagarooligosaccharide using the same 		Rep. of Korea 10-1919962
(13/11/2018)
5 Manufacturing method of agar for raw material of neoagarooligosaccharide mixture and use thereof Rep. of Korea 10-2214973
(04/02/2021)

Articles

  1. Anti-Obesity and Anti-Diabetic Effect of Neoagarooligosaccharides on High-Fat Diet-Induced Obesity in Mice. Marine drugs. 2017;15(4):90.
  2. Neoagarooligosaccharides enhance the level and efficiency of LDL receptor and improve cholesterol homeostasis. Journal of Functional Foods. 2017;38:529-539.
  3. Neoagarohexaose-mediated activation of dendritic cells via Toll-like receptor 4 leads to stimulation of natural killer cells and enhancement of antitumor immunity. BMB Reports. 2017;50(5):263-268.
  4. Neoagarooligosaccharides prevent septic shock by modulating A20- and cyclooxygenase-2–mediated interleukin-10 secretion in a septic-shock mouse model. BBRC. 486(4), 998-1004, 2017.
  5. Toxicological evaluation of neoagarooligosaccharides prepared by enzymatic hydrolysis of agar. Regulatory Toxicology and Pharmacology. 2017;90:9-21.
  6. In vitro and in vivo Investigation for Biological Activities of Neoagarooligosaccharides Prepared by Hydrolyzing Agar with β-Agarase. Biotechnology and Bioprocess Engineering. 2017;22:489-496.
  7. Safety evaluation of β-agarase preparations from Streptomyces coelicolor A3(2). Regulatory Toxicology and Pharmacology. 2019; 101, 142-155.
  8. Non-targeted metabolomics unravels a media-dependent prodiginines production pathway in Streptomyces coelicolor A3(2). PLoS ONE. 2018;13(11): e0207541.
  9. Implications of agar and agarase in industrial applications of sustainable marine biomass. Applied Microbiology and Biotechnology 2020;104:2815–2832.
  10. Hepatoprotective effect of neoagarooligosaccharide via activation of Nrf2 and enhanced antioxidant efficacy. Biol Pharm Bull. 2020;43(4), 619-628.
  11. Antiviral efficacy of orally delivered neoagarohexaose, a nonconventional TLR4 agonist, against norovirus infection in mice. Biomaterials 2020;263, 120391.
  12. Neoagarooligosaccharide Protects Against Hepatic Fibrosis via inhibition of TGF‐β/Smad Signaling Pathway. Int. J. Mol. Sci. 2021;22. 2041.