Description
Dyne RNA LoadingSTAR replace toxic Ethidium Bromide (EtBr), a potent mutagen, for the visualization of RNA in agarose and polyacrylamide gel electrophoresis.
Dyne RNA LoadingSTAR contains red color tracking dye and a safe, non-carcinogen red fluorescent dye.
Red dye is used for monitoring the agarose gel while it is running (approx 1 kb). During the gel electrophoresis, the agarose gel will reveal a red band.
Thus, the gel can be directly visualized under UV light without any extra staining steps.
In addition, RNA stained with the red fluorescent dye will emit a red light (595 nm) similar to Ethidium Bromide (EtBr).
Protocol
1. Mix your samples with the Dyne RNA LoadingSTAR in a 1:5 ratio. For example, add 1μl of the Dyne RNA
LoadingSTAR to 5 μl of RNA sample; or 2 μl of the Dyne RNA LoadingSTAR to 10 μl of RNA sample.
2. Run the agarose gel and visualize it under LED or UV light when ready. Take a picture as needed commonly
Components
● red dye, non-carcinogen red fluorescent dye
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